Spectators and participants in the pre-game show at Nationals Stadium on Tuesday took a moment to honor the victims of the mass shooting at the Pulse nightclub in Orlando, Fla. Members of the LGBT community carried a large rainbow flag onto the field as the Gay Men’s Chorus of Washington sang “True Colors” by Cyndi Lauper.

Team D.C., an umbrella organization for area LGBT-friendly sports clubs and leagues, held its annual Night Out at the Nationals on June 14. Gay former Major League Baseball player Billy Bean, who now serves as MLB’s ambassador of inclusion, was on the field alongside Bishop Gene Robinson.

The Gay Men’s Chorus of Washington sang the National Anthem with gay country singer Ty Herndon. “Will & Grace” actor Leslie Jordan threw out the first pitch wearing a “Make Baseball Fun Again” shirt.

Team D.C. Executive Director Brent Minor estimates that 3,500 people participated in the 12th annual Night Out at the Nationals.

Spectators posted videos to the Team D.C. Facebook page. Watch here: